Web7 grams of 3 wt% alginate, 2.1 mL of 100 mM MES buffer (pH 5) solution, 560 μL of 6-aminofluorescein (30 mg/mL of RO water) solution, 280 μL of EDC (50 mg/mL in 25 mM MES buffer) solution, and 280 μL of NHS (30 mg/mL of RO water) solution. All solutions were combined into a 20 mL vial and were stirred for 15 minutes.
Amine coupling through EDC/NHS: a practical approach
WebHybrid injectable and biodegradable hydrogels based on oxidized alginate/gelatin and containing nitrogen-doped carbon dots (NCDs) as a reinforcement have been fabricated and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) as the chemical crosslinking agents in the hydrogel system.The idea of composite … WebAlginate is highly biocompatible and has tunable physical properties that make it desirable for many biomedical applications, including gene and drug delivery [35, 141, 142]. … phosphine fmp
Can EDC/NHS chemistry be destructive for nanoparticles?
WebI agree with all above folks, specially, Chandan. I would suggest you to check pH of alginate particle solution before and after addition of EDC/NHS, to make sure the coupling pH (6~7) to be adjusted. Web1 day ago · A NIR- and pH-responsive injectable nanocomposite alginate-graft-dopamine hydrogel was prepared through the oxidative coupling of the catechol groups of SD and PFD catalytically cross-linked by the H 2 O 2 /HRP system to improve the efficacy against melanoma and promote skin repair (Scheme 1).The SD conjugates were synthesized by … WebDec 1, 2024 · Then, EDC (500 mg) and NHS (427.1 mg) added to the mixed solution. After stirring for 20 min, the corresponding amount of dopamine hydrochloride (Table 1) was … phosphine dot and cross diagram