T-stim with con a
WebT 细胞需要外部信号才能从静态状态下分化和扩增。伴刀豆球蛋白 A（con A）是一种抗原非依赖性丝裂原，可用作替代 T 细胞刺激物。在T细胞刺激实验中，这种凝集素常被用来替 … Web10% T-STIM with Con A (rat IL-2 culture supplement ) Becton Dickinson, Catalog No. 354115) 10% fetal bovine serum ; 0.05 mM 2-mercaptoethanol ; 10 pg/ml mouse IL-1 alpha (R&D Systems, Catalog No. 400ML) Temperature. 37°C. Atmosphere. 95% Air, 5% CO 2. Handling procedure
T-stim with con a
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WebT-STIM with ConA (IL-2 culture supplement), rat, used to promote proliferation and activation of T-cells and support high-titer HIV production by leukocytes. T-Cell Culture … WebApr 1, 2003 · Following appropriate stimulation, T lymphocytes will proliferate extensively in vitro. Traditionally, mitogenic lectins such as phytohemagglutinin (PHA) and concanavalin A (Con A) have been used for polyclonal T cell stimulation. A more physiologically relevant approach uses beads coated with anti- …
WebJan 22, 2024 · CultureT-STIM(IL-2),ratwithConA100ml . Brand: Corning - DISCOVERY LABWARE. Currently unavailable. We don't know when or if this item will be back in stock. Product details. Date ... WebFor culturing CTLL-2 cells, ATCC recommends to use CORNING T-STIM with Con A as a source for IL-2. However, delivery (for all distributors) would take almost two months and …
WebConcanavalin A (ConA) is a lectin (carbohydrate-binding protein) originally extracted from the jack-bean (Canavalia ensiformis).It is a member of the legume lectin family. It binds specifically to certain structures found in various sugars, glycoproteins, and glycolipids, mainly internal and nonreducing terminal α-D-mannosyl and α-D-glucosyl groups. WebCon A (5 Ag/ml), whereas lower levels were achieved using one bead per lymphocyte (Fig. 1). The rate of T lymphocyte expansion and hence the total number of cells produced …
WebT cells require external signals for differentiation and expansion from a quiescent state. Concanavalin A (con A) is an antigen-independent mitogen and can be used as an …
WebT-STIM with Con A (rat IL-2 culture supplement from Becton Dickinson) may be used or the rat factor may be prepared as described on the product sheet. The freshly prepared rat … biomes o plenty just ocean worldWebUsually dispatched in 14-28 days. BD Rat t-stim with con a 10000 units. Scientific Laboratory Supplies 4titude Standard 96 Well qpcr Plate Non-Skirted Green Polypropylene. Unit Price. … daily sentinel grand junction co obituariesWebT-STIM with ConA (IL-2 culture supplement), rat, is used to promote proliferation and activation of T-cells and support high-titer HIV production by leukocytes.The tissue culture media supplements and additives compensate for characteristics the base medium lacks … biomes o plenty oasisdaily sentinel obituaries rome nyWebOne of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This … daily sentinel pomeroy ohio 1966WebT cells require external signals for differentiation and expansion from a quiescent state. Concanavalin A (con A) is an antigen-independent mitogen and can be used as an alternative T cell stimulus. This lectin is frequently used as a surrogate for antigen-presenting cells in T cell stimulation experiments. daily sentinel grand junction obituariesWebThese two types of stimulation use very different pathways. More importantly, Anti-CD3 is specific for T-cells whereas ConA stimulates many different cells and promote … biomes o plenty nether wasp